Protocolo de superovulación con hormona menopáusica humana y transferencia de embriones en cobayas

Abstract

The present study evaluated the efficacy of human menopausal hormone (hMG) to induce superovulation in guinea pigs and explored the in vivo embryo transfer technique. The research was carried out in a commercial farm located in Baños, Cuenca canton, at an altitude of 2,678 meters above sea level and an average temperature of 14°C. The collection and transfer of embryos was carried out at the Faculty of Agricultural Sciences of the University of Cuenca. The work was done with 10 donor guinea pigs (G1) and 5 recipients (G2). The weight and body condition (BC) of the donors were in accordance with a female with adequate sexual maturity, mainly because they presented a high BC score, which favored the response to SOV superovulation (70%), reaching 72% ovulations and a recovery of 36% structures. Of the total number of donors (n=10), 7 showed a positive response to the SOV protocol, of which 11 embryos were collected: 10 early morulas and 1 blastocyst, with an average of 1.5 embryos per wash. The low recovery rate was attributed to inaccuracies in determining the day of mating, which affected the determination of the location and development of the embryos. Although the postoperative monitoring of the physiological parameters was stable, 2 recipients died due to peritonitis. The survivors showed adequate healing; but no pregnancies were achieved; however, one of the recipients showed signs of embryo resorption. This innovative study highlights the importance of perfecting synchronization methods, as well as improving the different embryo transfer techniques, to increase reproductive efficiency in future research in guinea pigs