Efecto del Ácido Naftalenacético (ANA) y la Bencilaminopurina (BAP) en el desarrollo de micro-esquejes de tomate de árbol (Solanum betaceum Cav.) in vitro

Abstract

Tree tomato (Solanum betaceum Cav.) is a fruit tree of commercial importance in Ecuador, especially in the provinces of the Ecuadorian highlands. Propagation is carried out by traditional methods, which generates inconveniences in the development and production of the plant. It allows looking for efficient methods to obtain healthy plants. The objective of this study was to evaluate the effect of Naftaleneacetic acid (NAA) and Benzylaminopurine (BAP) on the development of micro-scions of tree tomatoes (Solanum betaceum Cav.) in vitro. For this, young plants were selected, and nine treatments were applied, which were T1= 0 ppm ANA+0 ppm BAP, T2= 0 ppm ANA+0.25 ppm BAP, T3= 0.5 ppm ANA+0 ppm BAP, T4= 0.25 ppm ANA+0.13 ppm BAP, T5= 0.5 ppm ANA+0. 25 ppm BAP, T6= 0.75 ppm ANA+0.38 ppm BAP, T7= 0.13 ppm ANA+0.25 ppm BAP, T8= 0.25 ppm ANA+0.5 ppm BAP and T9= 0.38 ppm ANA+0.75 ppm BAP, on Phytamax 75% culture medium and solidified with 7.5 g/L agaragar. The experimental unit consisted of a micro-shoot to which one treatment was applied. The variables to be measured were leaf number, root length (mm), shoot length (mm) during the 90 days, shoot and root biomass at 90 days. In addition, micro-shoot thickening was recorded for each treatment. The variables shoot length and number of leaves were used for data analysis. Treatment two with 0.5 ppm BAP favored shoot length (4.5 mm), in contrast to the ANA+BAP combination that promoted 60% shoot thickening without the presence of shoots.