Comparación de eficiencia en los métodos de fraccionamiento para microalgas y/o cianobacterias a través de la evaluación de actividad antioxidante

Abstract

Due to the interest in the search for antioxidants from natural sources, microalgae and cyanobacteria have attracted attention for their ability to synthesize bioactive compounds under extreme conditions of survival. So, the study of biodiversity and biotechnological applications conferred by this group of microorganisms is of great importance for science. In this research, the review of two methods of extraction of bioactive components for microalgae and cyanobacteria obtained from species cultivated in the Microalgae Research Laboratory of the VLIR Group of "Medicinal Plants" of the Department of Biosciences of the University of Basin. Indeed, micritization and ultrasound were compared, techniques that promote cellular fragmentation and the release of intracellular material. In turn, the antioxidant capacity of the released bioactive content was determined by applying the DPPH (2,2-diphenyl-1-picrylhydrazyl) technique for each methanolic extract of the three analyzed species: Tamariz # 6, Tamariz # 11 and Flores # 1. The DPPH technique assessed radical discoloration (DPPH) by the action of an antioxidant (methanolic extract). This evaluation was expressed through the calculation of the AAEAC (milligrams of ascorbic acid equivalent to 100 grams of dry drug) reaching values between 14.53 mg / g - 20.08 mg / g for extracts treated by the Ultrasound method and values between 66.20 mg / g - 73.17 mg / g for those of the Micronization method. The result of this study determined that the most efficient technique for extraction of bioactive components was by the micronization method because it revealed higher antioxidant activity due to the large amount of bioactive components present in the extracts.