Evaluación del estado microbiológico de la central de mezclas del hospital Vicente Corral Moscoso de la ciudad de Cuenca

Abstract

The parenteral nutrition is a therapeutic technique widely used to provide nutrients effectively to incapacitated patients receiving their nutrition requirements orally. The preparations that are elaborated in the “Vicente Corral Moscoso “hospital are considered sterile master preparations of medium risk, so it should be developed in an area that meets the right characteristics to reduce the contamination risks and ensure the quality of the preparation. It is also considered that all the staff who make such preparations should be trained to ensure the asepsis and quality of the final product. Objective: assess the microbiological status of the parenteral nutrition, environment and the technique used by staff. Methodology: The analysis was carried out following the indications given by the United States Pharmacopeia, in chapter 797 for sterile preparations. For parenteral nutrition the sample was directly sown in a tube of trypticase soy broth and it was incubated for 24 hours, after this time it was sowed again in blood agar to assess the sterility of the preparations. In the analysis of the environment the method of sedimentation in tripticase soy agar plates was used. To assess the aseptic technique, a simulation of the preparation of a parenteral nutrition was performed. Results: In the control of the parenteral nutrition and the aseptic technique, no growth of any microorganism was obtained, checking their sterility. Microbial contamination by Micrococcus spp. was evidenced in 11,6% of the samples evaluated in environments. The growth of this microorganism does not exceed 1 colony forming unit, which is within the permitted limit for sterile areas.