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Title: Effect of resveratrol supplementation in conventional slow and ultra-rapid freezing media on the quality and fertility of bull sperm
Authors: Galarza Lucero, Diego Andres
Jaramillo Lopez, Jennifer Alexandra
Amon Togra, Nicole Alexandra
Aguirre Narea, Brian Oswaldo
Campoverde Guailacela, Byron Alexander
Taboada Pico, Juan Wualverto
Samaniego Campoverde, Jorge Xavier
Duma Pauta, Jose Mauricio
metadata.dc.ucuenca.correspondencia: Galarza Lucero, Diego Andres, andres.galarza@ucuenca.edu.ec
Keywords: Resveratrol
Bull sperm
Conventional slow freezing
Ultra-rapid freezing
metadata.dc.ucuenca.areaconocimientofrascatiamplio: 4. Ciencias Agrícolas
metadata.dc.ucuenca.areaconocimientofrascatidetallado: 4.3.1 Ciencias veterinarias
metadata.dc.ucuenca.areaconocimientofrascatiespecifico: 4.3 Medicina Veterinaria
metadata.dc.ucuenca.areaconocimientounescoamplio: 08 - Agricultura, Silvicultura, Pesca y Veterinaria
metadata.dc.ucuenca.areaconocimientounescodetallado: 0841 - Veterinaria
metadata.dc.ucuenca.areaconocimientounescoespecifico: 084 - Veterinaria
Issue Date: 2024
metadata.dc.ucuenca.volumen: Volumen 266
metadata.dc.source: Animal Reproduction Science
metadata.dc.identifier.doi: 10.1016/j.anireprosci.2024.107495
metadata.dc.type: ARTÍCULO
Abstract: 
The study investigated the impact of resveratrol (RES) on bull sperm cryopreservation employing conventional slow (CS) and ultra-rapid (UR) freezing methods on sperm quality and in vitro fertility. Twenty-four ejaculates from four bulls were divided into four groups based on the cryopreservation method and RES addition: CS-RES (n = 80), CS-Co (n = 80), UR-RES (n = 24), and UR-Co (n = 24). The CS freezing involved exposing sperm straws with 5% glycerol to liquid nitrogen (LN2) vapors, while UR freezing submerged sperm drops with 100 mM sucrose directly into LN2. Overall, sperm kinematic parameters and integrity of plasma and acrosome membranes significantly decreased (P < 0.001) after cryopreservation. Post-thaw values of motilities (total [TM] and progressive [PSM]), velocities (curvilinear and straight-line), beat cross frequency (BCF), and sperm with intact plasma membrane/intact acrosome (PI-/PNA-) were higher (P < 0.05) with CS-RES and CS-Co treatments compared to UR-RES and UR-Co treatments. CS-RES treatment resulted in greater percentages (P < 0.05) of TM, PSM, PI-/PNA-, and fertility (blastocyst rate) than their control, CS-Co; while UR-RES showed higher BCF values (P < 0.05) than its control, UR-Co. Additionally, UR-RES treatment exhibited lower oxidative stress percentages than UR-Co (P < 0.05). This study presents the following conclusions: (1) the CS freezing resulted in better cryosurvival of bull sperm than UR freezing; (2) the RES supplementation to CS freezing medium improved sperm motility, membrane integrity, and fertility; and (3) despite low cryosurvival sperm and fertility, the RES addition to ultra-rapid freezing medium reduced oxidative stress.
URI: https://www.scopus.com/record/display.uri?eid=2-s2.0-85194043534&origin=resultslist&sort=plf-f&src=s&sid=882eaa7097690c2c7550116ee81d5399&sot=b&sdt=b&s=TITLE-ABS-KEY%28Effect+of+resveratrol+supplementation+in+conventional+slow+and+ultra-rapid+freezing+media+on+the+quality+and+fertility+of+bull+sperm%29&sl=147&sessionSearchId=882eaa7097690c2c7550116ee81d5399&relpos=0
metadata.dc.ucuenca.urifuente: https://www.sciencedirect.com/journal/animal-reproduction-science
ISSN: 0378-4320, e 1873-2232
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