Efecto del Resveratrol y L-carnitina suplementado al medio de maduración sobre la producción in vitro de embriones bovinos

Abstract

Resveratrol (RES) and L-carnitine (LC) are two effective additives/antioxidants for reducing oxidative stress and stimulating cellular energy metabolism. Accordingly, this research evaluated the effect of supplementing with LC (0.3 mg/ml), RES (1 μM), and their combination (RES+LC= 0.5 μM + 0.15 mg/ml) to the MIV medium of oocytes on the rate of nuclear maturation (MII) and the rate of embryonic development (cleavage and blastocysts) following in vitro fertilization (IVF). A total of 1209 oocytes were recovered from bovine ovaries and matured in vitro in MIV medium supplemented with LC (n = 304), RES (n = 300), LC+RES (n = 302), and control (n = 303). After 24 hours of incubation, a batch of 100 oocytes from each group was assessed for nuclear maturation (MII), and the remainder were fertilized and cultured in vitro for 7 days. The results demonstrated no significant differences in the MII rate (P>0.05). The cleavage rate was higher (P<0.05) in oocytes matured with LC compared to the control. The blastocyst rate was significantly higher (P<0.05) in oocytes matured with LC than those matured with LC+RES and control (39.7 ± 3.43% vs. 27.2 ± 3.14% and 30.0 ± 3.23%, respectively). In conclusion, L-carnitine supplementation to the maturation medium of bovine oocytes resulted in improved embryonic development, whereas resveratrol, either alone or combined with L-carnitine, had no significant effect on the in vitro production of bovine embryos