A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.

Parkhouse, Michael; Campoverde Cisneros, Manuel Alfredo; Harrison, Leslie; Carpio Rodas, Luis Arturo; Cortéz, María Milagros; Rojas, Glenda C.; Sastre, Patricia

Please use this identifier to cite or link to this item: https://dspace.ucuenca.edu.ec/handle/123456789/42383

Title:	A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.
Authors:	Parkhouse, Michael Campoverde Cisneros, Manuel Alfredo Harrison, Leslie Carpio Rodas, Luis Arturo Cortéz, María Milagros Rojas, Glenda C. Sastre, Patricia
metadata.dc.ucuenca.correspondencia:	Parkhouse, Michael, parkhous@igc.gulbenkian.pt
Keywords:	Neurocysticercosis (NCC) Field diagnosis Bovine cysticercosis HP10 antigen ELISA HP10 antigen LFA
metadata.dc.ucuenca.areaconocimientofrascatiamplio:	3. Ciencias Médicas y de la Salud
metadata.dc.ucuenca.areaconocimientofrascatidetallado:	3.3.1 Ciencias y Servicios del Cuidado de la Salud
metadata.dc.ucuenca.areaconocimientofrascatiespecifico:	3.3 Ciencias de la Salud
metadata.dc.ucuenca.areaconocimientounescoamplio:	09 - Salud y Bienestar
metadata.dc.ucuenca.areaconocimientounescodetallado:	0912 - Medicina
metadata.dc.ucuenca.areaconocimientounescoespecifico:	091 - Salud
Issue Date:	2019
metadata.dc.ucuenca.embargoend:	29-Jul-2050
metadata.dc.ucuenca.volumen:	vol. 113
metadata.dc.source:	Transactions of the Royal Society of Tropical Medicine and Hygiene
metadata.dc.identifier.doi:	10.1093/trstmh/try116.
metadata.dc.type:	ARTÍCULO
Abstract:	Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was …
Description:	Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was …
URI:	http://dspace.ucuenca.edu.ec/handle/123456789/42383 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061121773&origin=inward
metadata.dc.ucuenca.urifuente:	http://www.sciencedirect.com/science/journal/00359203
ISSN:	0035-9203
Appears in Collections:	Artículos

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