Evaluación de los sistemas de cultivo semisólido y BIT en la multiplicación in vitro de Juglans neotrópica

Abstract

The purpose of this study was to evaluate two culture systems; semisolid and temporary immersion in bioreactors (BIT); for the micropropagation of Juglans neotropica;using zygotic embryos Additionally; in the BIT culture system also the effect of the frequency of immersion and forced aeration on the micropropagation was examined The culture growth of the shoots was measured by the following characteristics number and size of the shoots; number of leaves; and the wet and dry weight No statistical differences were detected In general; the BIT immersion system produced an equivalent number of plants of similar characteristics as the embryos propagated in the traditional semisolid medium method However; BITs produced more shoots; forced ventilation resulted in less hyperhydric shoots and the BIT system was considerable cheaper; resulting in an average production cost of $0.21 per plant versus $0.40 in the semisolid propagation method.