Relación entre niveles de hormona antimulleriana y recuento de folículos antrales en vaquillas Holstein mestizas pre y pos faenamiento

Abstract

The aim of the present study was to determine the relationship between pre- mortem Anti-mullerian hormone (AMH), antral follicle count (RFA) and post- mortem ovarian morphology, in crossbred Holstein heifers that were slaughtered in slaughterhouses from Cañar and Azuay provinces. In the first instance before to slaughter, 4 ml of blood was taken by coccygeal venipuncture to measure plasma AMH levels, using the DL-AMH-b kit (Develop®). Post mortem, the ovaries were recovered from the heifers previously assessed. Two methods were performed for antral follicular count (RFA) and measurement of the ovaries. The first utilizing an ultrasound scanner (Prosound 2), with a linear transducer, 7.5 Mhz and the second by direct vision; classifying them into 2 groups <4mm and> 4mm. The classification was made in groups of high and low AMH levels. The general average AMH value obtained was 0.08 ± 0.006 ng / ml. When evaluating RFA <4mm, an average of 16.76 ± 1.34 follicles was determined in the group (low) and 37.66 ± 2.48 follicles in the group (high), with a significant difference (P<0.05 ); This was also observed in the RFA by the ultrasound method in the RFA <4mm, establishing an average of 20.94 ± 1.14 follicles (high) and 11.46 ± 0.95 follicles (low), results that differ from RFA> 4mm with an average of 7.36 ± 0.47 follicles (high), and 5.83 ± 0.37 follicles (low), without a difference (P> 0.05). In the morphological characteristics, a significant difference (P <0.05) was found in the size of the right ovary (24.2 ± 0.67mm) that was greater in the high group, compared to the low group (21.7 ± 0, 47mm). As in the left ovary, the high group (21.2 ± 0.53mm) and the low group (18.9 ± 0.44mm). A similar trend was observed in the weight of the right ovary in the high group (9.07 ± 0.62g) and low (7.42 ± 0.42g). However, when the corpus luteum size was compared in the high (15.4 ± 0.64mm) and low (14.1 ± 0.66mm) groups, similar values were observed (P> 0.05). As a conclusion, a positive and significant correlation was found between the levels of AMH evaluated (pre-mortem) and the RFA in the ovaries assessed directly post-mortem.