Validación del método para determinar materia orgánica en el suelo por la técnica de Walkley and Black

Abstract

This work aims to objective validate the method of analysis of organic matter in soils by the Walkley Black technique. Currently the soil laboratory of the faculty of Chemical Sciences of the University of Cuenca has set validation objectives for analysis, so a method to validate is the organic matter under the recommendations of the accreditation institutes for compliance with the standard NTE INEN-ISO/IEC 17025:2005, but this work must prove that the method used is adjusted to their requirements, to obtain reliable and accurate results. In the soil analysis laboratory they made a series of tests that confirm the technical characteristics of the method used in the analysis such as repeatability, reproducibility, detection limit, quantification limit and ANOVA analysis. Also, the results obtained in the validation of the analytical method proposed have been compared with the results of another laboratory of soil analysis with validated techniques and methods and that this be accredited. On the other hand, due to the limitations of working time, the calculation of uncertainty is excluded. The results of analysis together with the objectives established in this work indicate if the test method for the determination of Organic Matter in a soil sample by the Walkley Black technique is declared successfully validated.