Asociación genotípica y fenotípica del polimorfismo 2548G>A del gen LEP con diabetes Mellitus tipo 2 en pacientes ambulatorios de la Fundación Donum

Abstract

The present research is a non-experimental cross-sectional study with case and control design. The aim of the study was to genotypic and phenotypically association of the SNP 2548G>A of the LEP gene with the presence of type 2 diabetes mellitus in outpatients of the DONUM Foundation. A total of 116 patients were enrolled and divided into two equal groups (n = 58), according to the previous diagnosis of DM2, the groups were paired by age, gender and body mass index. The history of cardiovascular, cerebrovascular, renal diseases, immunodeficiency diseases; and gestation status were exclusion criteria. Data collection was done through the direct interview using a survey; EDTA tubes were used to collect blood samples; later, it was performed the DNA extraction, and the genetic material was amplified using the real time PCR technique. The Statistical analysis was performed with 95% confidence interval and p <0.05. The presence of the A allele and the AA genotype was found with higher frequency (16%) in the DM2 group, compared with the controls (12%); however, no statistically significant association was found between the presence of the allelic variant and the development of Type 2 Diabetes Mellitus.